Homebase's St Austell branch is the latest to join the growing list of the retailers' stores earmarked for closure.
The company has entered into a consultation period with staff at the Cornish store.
A spokesperson said: "Homebase can confirm that the business has entered a period of consultation with colleagues concerning the proposed closure of our store in St Austell.
"Colleagues at the store have been informed and we will be working with them over the next few weeks to support them as much as possible.
"Due to commercial sensitivities, we are unable to comment further at this time."