The jobs will be cut from B&Q's head office as the company looks to transform itself into an omnichannel retailer.
Explaining the redundancies a spokeswoman for the company said: "To meet the needs of our business, our employee levels continually fluctuate as new roles are added and others are removed."
It is understood that around 1,200 staff are currently employed in B&Q's head office in Eastleigh, Hampshire.
The news comes as part of B&Q boss, Kevin O'Byrne's plans to simplify the business structure. Mr O'Byrne took over from former chief executive Martyn Phillips in October last year.
Diyweek.net reported in August that 180 redundancies were made under a management restructure and a further 220 head office staff lost their jobs in October 2012.